Manganese (Mn) is a key cofactor in enzymes responsible for lignin decay (mainly Mn peroxidase), regulating the rate of litter degradation and carbon (C) turnover in temperate and boreal forest biomes.While soil Mn is mainly derived from bedrock, atmospheric Mn could also contribute to soil Mn cycling, especially within the surficial horizon, with implications for soil C cycling. However, quantification of the atmospheric Mn cycle, which comprises emissions from natural (desert dust, sea salts, volcanoes, primary biogenic particles, and wildfires) and anthropogenic sources (e.g. industrialization and land-use change due to agriculture) transport, and deposition into the terrestrial and marine ecosystem, remains uncertain. Here, we use compiled emission datasets for each identified source to model and quantify the atmospheric Mn cycle with observational constraints. We estimated global emissions of atmospheric Mn in aerosols (<10 µm in aerodynamic diameter) to be 1500 Gg Mn yr-1. Approximately 32% of the emissions come from anthropogenic sources. Deposition of the anthropogenic Mn shortened soil Mn “pseudo” turnover times in surficial soils about 1-m depth (ranging from 1,000 to over 10,000,000 years) by 1-2 orders of magnitude in industrialized regions. Such anthropogenic Mn inputs boosted the Mn-to-N ratio of the atmospheric deposition in non-desert dominated regions (between 5×10-5 and 0.02) across industrialized areas, but still lower than soil Mn-to-N ratio by 1-3 orders of magnitude. Correlation analysis revealed a negative relationship between Mn deposition and topsoil C density across temperate and (sub)tropical forests, illuminating the role of Mn deposition in these ecosystems.

Microbial processing of fresh carbon inputs is recognized as a key step in the formation of mineral-associated organic matter. Low molecular weight (LMW) compounds comprise a notable fraction of these inputs and are rapidly assimilated and metabolized by the microbial community. In this work, we employ ecophysiological studies of microbial isolates to better understand the role of substrate identity as a control on preferences, uptake kinetics, and carbon use efficiencies (CUE) across a gradient of phylogenetic differences (gram negative, gram positive, and fungal). Soil-extracted, solubilized organic matter (SESOM) derived from the Oa horizon of a hemlock-hardwood forest stand and synthetic media based off of this extract were chosen as liquid media for batch growth studies. A combination of exometabolomic techniques (1H NMR, UHPLC-MS) were used to quantify 35 LMW substrates in the original extract (0.4 – 195 μM), comprising 19.5% of total C and 39.9% of total N. Consumption of these substrates by microbial isolates accounted for a substantial amount of total C and N assimilated during growth, representing 43-75% and 58-74%, respectively. Time resolved sampling allowed modeling of sigmoidal uptake curves and the comparison of the midpoint of consumption (Th, hr) and 90% usage windows (ranging from 0.18 – 2.29 hr). Complementary experiments were conducted using synthetic media with all substrates at equimolar concentrations (25 μM) to better constrain the impact of initial concentration. We use stable isotope probing to determine CUE for five different LMW substrates of interest (glucose, acetate, formate, glycine, and valine). Ultimately, we are interested in whether unifying trends can be observed across the physiological gradient and how the metabolic transformations of these inputs may impact the organo-mineral formation process.