Molecular techniques like metabarcoding, while promising for exploring diversity of communities, are often impeded by the lack of reference DNA sequences available for taxonomic annotation. Our study explores the benefits of combining targeted DNA barcoding and morphological taxonomy to improve metabarcoding efficiency, using beach meiofauna as a case study. Beaches are globally important ecosystems and are inhabited by meiofauna, microscopic animals living in the interstitial space between the sand grains, which play a key role in coastal biodiversity and ecosystem dynamics. However, research on meiofauna faces challenges due to limited taxonomic expertise and sparse sampling. We generated 775 new cytochrome c oxidase I DNA barcodes from meiofauna specimens collected along the Netherlands’ west coast and combined them with the NCBI GenBank database. We analysed alpha and beta diversity in 561 metabarcoding samples from 24 North Sea beaches, a region extensively studied for meiofauna, using both the enriched reference database and the NCBI database without the additional reference barcodes. Our results show a 2.5-fold increase in sequence annotation and a doubling of species-level Operational Taxonomic Units (OTUs) identification when annotating the metabarcoding data with the enhanced database. Additionally, our analyses revealed a bell-shaped curve of OTU richness across the intertidal zone, aligning more closely with morphological analysis patterns, and more defined community dissimilarity patterns between supralittoral and intertidal sites. Our research highlights the importance of expanding molecular reference databases and combining morphological taxonomy with molecular techniques for biodiversity assessments, ultimately improving our understanding of coastal ecosystems.

In 2015, Integrated Ocean Discovery Program Expedition 356 drilled along the margin off Western Australian to investigate the history of the Indonesian Throughflow (ITF) and its integral role in the development of global thermohaline circulation and climate. Throughout the expedition, a suite of foraminiferal analyses were employed wherein planktic specimens provided biostratigraphy and an incredibly diverse benthic fauna (~ 260 species) was used to reveal palaeo- water depth, palaeobathymetric setting and variable conditions at the sediment-water interface. Benthic foraminiferal biofacies are particularly sensitive to changes in environmental conditions, have a rapid turnover and are ideal proxies for monitoring physical and chemical changes in marine environments. When this information is combined with lithostratigraphic and other microfossil data, a robust understanding of past environments and past geological events can be reconstructed. Shipboard data were used to isolate horizons of interest for more intense sampling at IODP Site U1461, situated on the North-West Shelf, at 127 m of water depth. The shipboard data revealed a large (~150 m-thick) turbidite horizon hosting benthic foraminifera from a substantially shallower water depth than the horizon immediately preceding the horizon. We present preliminary foraminiferal results combined with shipboard sedimentary descriptions to better constrain the deposit’s occurrence in the biostratigraphic record, use benthic foraminifera to elucidate the deposit’s sedimentary origins and link this event with others in the region to investigate potential catalysts for its deposition.