Climate-induced episodes of extensive tree mortality worldwide are leading to abrupt changes in forest carbon stocks. A severe frost in early February 2011 triggered widespread tree mortality in the lowland tropical dry forest (TDF) of northwestern Mexico. The studied landscape in southern Sonora is composed by a patchy matrix dominated by mature, secondary (originated in abandoned fields), and active agricultural fields. In this forest, we used allometric equations to assessed frost-induced changes in aboveground biomass (AGB) stocks in mature and secondary tropical dry forests. For AGB estimations we used 48 1-ha plots (24 plots per forest type) distributed within four distant subareas in our 83 230 ha study area. On each plot, we recorded all live/dead individuals, and a total of 11 205 woody plants were registered, of which 7 137 (with at least a stem DBH > 2.5 cm) were likely present before the frost, and the remaining smaller ones were considered as new recruits regenerated from seeds (4,068 individuals). From those plants present before the frost, 26 842 and 8 059 were live and dead stems, and 1 222 were dead individuals. All registered live and dead stems accounted for a total of 273.4 Mg of AGB in our study plots (4.8 ha). From this amount, 57.3 Mg was necromass (dead stems). Interestingly, only two out of a total of 74 registered species contributed with ca. 80% of this necromass. These highly sensitive species are the tree legumes Lysioma divaricatum and Acacia cochliacantha. On average, AGB in the studied mature and secondary TDF was 64.3 and 49.6 Mg ha-1, respectively. The corresponding necromass for these forests was 10.9 and 13 Mg ha, respectively. The 2011 frost induced a greater change from live biomass to necromass in secondary than mature forests, 26.2% and 16.9%, respectively, which can be explained by the higher abundance of individuals from sensitive species in secondary forests. Our results suggest that climate-induced shifts in carbon stocks are linked to previous land-use changes in tropical dry forests. Restoration plans of these degraded lands should consider the vulnerability of tropical dry forest species to climate extremes.

Forests are under major pressures due to contemporary land-use, which creates mosaics of stand-stage development that follow different successional paths, that imply ecosystem complexity. The interplay of carbon and water dynamics across succession involves physical and biological interactions that shape net ecosystem production (NEP) and water use efficiency. Here we present 13 years-site of eddy covariance data (2016-2020) from a seasonally dry tropical forest in Northwestern Mexico to elucidate the environmental controls on ecosystem fluxes and explore the interactions with changes in resource availability. Across a successional gradient, an early (9 years since abandonment) and a mid-successional (about 45 years with natural recruitment and regrowth) sites were net carbon sinks (in the order of 100 to 500 g C m-2 y-1) while an old- growth forest was a chronic net source over the 5 years studied (losing between 100 and 300 g C m-2 y-1). In contrast evapotranspiration was alike at sites and close to the precipitation input. Ecosystem water use efficiency tended to be higher at the old-growth forest site (ca. 3.0 g C m-2 /mm H2O vs. ca. 2.0 g C m-2 / mm H2O at the secondary sites). Water availability and radiation where clearly dominant environmental controls across sites, but notably vapor pressure deficit was not a controlling factor for gas exchange at the old-growth forest. Surface characteristics, canopy structure and species composition may explain differences in NEP across succession in TDF at its northernmost extent.