The role of hybrid zones on the phylogeography of two willow species
pairs (Salix L.) in the European Alps
- Loïc Pittet,
- Pia Marinček,
- Piotr Kosiński,
- Natascha Wagner,
- Elvira Hörandl
Pia Marinček
University of Göttingen Albrecht-von-Haller-Institute for Plant Sciences
Author ProfilePiotr Kosiński
Institute of Dendrology Polish Academy of Sciences
Author ProfileNatascha Wagner
University of Göttingen Albrecht-von-Haller-Institute for Plant Sciences
Author ProfileElvira Hörandl
University of Göttingen Albrecht-von-Haller-Institute for Plant Sciences
Author ProfileAbstract
Pleistocene climate oscillations influenced the biogeographical history
of most species. In the European Alps, mountain plants were restricted
to refugial areas during cold phases of glacial cycles and recolonized
newly available habitats during warm periods. The current ranges of
alpine plants represent a transient stage of a continuous and dynamic
recolonization process that started after the last glaciations.
Differences in recolonization rate and range filling are observed
between different mountain plant species, but the reasons remain
insufficiently explored. Here, we investigated the effects of secondary
contact hybridization on range expansion between two related willow
species pairs that came into secondary contact. RAD sequencing data was
used to identify potential refugial areas and characterize the secondary
contact zones. Leaf phenotypes were measured using morphometrics.
Distribution modeling was used to find current suitable habitats.
Results suggests that peripheral glacial refugia played a major role in
the history of the species. For both species' pairs, the secondary
contact zones showed homoploid hybridization between parents, which is
also supported by the morphometric analyses. The hybrid zones are
broader than expected and characterized by introgression. Current
projections of species distribution identified suitable habitats beyond
the secondary contact zone. We suggest that the parents' range expansion
is blocked by the hybrid zones. Indeed, due to the high genetic
similarity, each dispersal beyond the secondary contact zone results in
the long term in hybridization and introgression with the already
established parent. Therefore, hybridization acts here as a barrier to
further recolonization.