loading page

Comparison Study of Stem Cell-Derived Extracellular Vesicles for Bone Tissue Engineering
  • +1
  • Elham Pishavar,
  • Joshua Copus,
  • Anthony Atala ,
  • Sang Jin Lee
Elham Pishavar
Wake Forest School of Medicine

Corresponding Author:[email protected]

Author Profile
Joshua Copus
Wake Forest School of Medicine
Author Profile
Anthony Atala
Wake Forest School of Medicine
Author Profile
Sang Jin Lee
Wake Forest School of Medicine
Author Profile

Abstract

Stem cell-derived extracellular vesicles (EVs) have shown great promise in the field of regenerative medicine and tissue engineering. Recently, human bone marrow-derived mesenchymal stem cell (BMSC)-derived EVs have been considered for bone tissue engineering applications. In this study, we evaluated the osteogenic capability of placental stem cell (PSC)-derived EVs and compared them to the well-characterized BMSC-derived EVs. EVs were extracted from three designated time points (0, 7, and 21 days) after osteogenic differentiation. The results showed that the PSC-derived EVs had much higher protein and lipid concentrations than EVs derived from BMSCs. The extracted EVs were characterized by observing their morphology and size distribution before utilizing next-generation sequencing to determine their miRNA profiles. It was identified that 306 miRNAs within the EVs, of which 64 were significantly expressed in PSC-derived EVs that related to osteogenic differentiation. In vitro osteogenic differentiation study indicated that the late-stage (21-day extracted) derived EVs higher osteogenic enhancing capability when compared to the early-stage derived EVs. We demonstrated that EVs derived from PSCs could be a new source of EVs for bone tissue engineering applications.
02 Jul 2020Submitted to Biotechnology Journal
06 Jul 2020Submission Checks Completed
06 Jul 2020Assigned to Editor