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Layer-by-layer Adhesion of Hydrogels for Constructing Heterogeneous Microfluidic Chips
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  • Chong Shen,
  • Yingjun Li,
  • Wenqi She,
  • Qin Meng
Chong Shen
Zhejiang University
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Yingjun Li
Zhejiang University
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Wenqi She
Zhejiang University
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Qin Meng
Zhejiang University

Corresponding Author:mengq@zju.edu.cn

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Hydrogel-based microfluidics offer an in vivo-relevant micro-environments for construction of organs-on-chips. However, the fabrication of heterogeneous microchannels using hydrogels is challenging and fails to mimic the complex structures of organs in vivo. Here we present a new methodology called “layer-by-layer adhesion” for the construction of complex microfluidic chips. A hydrosoluble and photo-crosslinkable adhesive, chitosan methacryloyl (CS-MA), was used to stitch various hydrogels together layer-by-layer to form perfusable microchannels. Our results show that CS-MA can bond different types of hydrogels with adhesion energy ranging from 1.2-140 N/m. Using the layer-by-layer adhesion approach, we constructed heterogeneous hydrogel-based microchannels with various morphologies of snail, spiral, vascular-like, and bilayer. Based on this methodology, liver-on-a-chip was established by entrapping hepatic cells inside a biocompatible Gel-MA layer and covering it with the perfusable microchannels in tough F127-DA layer. The “layer-by-layer adhesion” provides a facile and cytocompatible approach for engineering user-defined hydrogel-based chips potentially for organs-on-chips.
09 May 2023Submitted to AIChE Journal
27 May 2023Submission Checks Completed
27 May 2023Assigned to Editor
27 May 2023Review(s) Completed, Editorial Evaluation Pending
31 May 2023Reviewer(s) Assigned
15 Jul 2023Editorial Decision: Revise Major
01 Aug 20231st Revision Received
06 Aug 2023Submission Checks Completed
06 Aug 2023Assigned to Editor
06 Aug 2023Review(s) Completed, Editorial Evaluation Pending
08 Aug 2023Reviewer(s) Assigned