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Purification of polyclonal Immunoglobulin G from human serum using LigaGuardTM and LigaTrapTM adsorbents
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  • Wenning Chu,
  • Hannah Reese,
  • Dipendra Bhandari,
  • Jae Sly,
  • Michael Crapanzano,
  • Stefano Menegatti
Wenning Chu
North Carolina State University

Corresponding Author:[email protected]

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Hannah Reese
North Carolina State University
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Dipendra Bhandari
LigaTrap Technologies LLC
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Jae Sly
LigaTrap Technologies LLC
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Michael Crapanzano
LigaTrap Technologies LLC
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Stefano Menegatti
North Carolina State University
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This study presents the chromatographic purification of immunoglobulin G (IgG) from human plasma using a two-column process integrating the peptide-based adsorbents LigaGuardTM, which captures non-Ig plasma proteins in flow-through mode, and LigaTrapTM, which isolates IgG in bind-and-elute. Buffer composition and column loading were optimized for both adsorbents. Two process configurations were evaluated. In the first design, plasma was fed to a LigaGuardTM column to capture plasma proteins, the effluent was loaded on the LigaTrapTM column, and the bound IgG was eluted with 63.8% global recovery and 99.7% purity; in comparison, Protein G agarose afforded ~67% recovery and 97.2% purity. In the alternative design, the LigaGuardTM column was utilized to polish the LigaTrapTM elution stream, affording 82.3% global recovery and 98.8% purity. Collectively, these results demonstrate the potential of a fully chromatographic process for purifying polyclonal IgG from plasma feedstocks.
25 Jun 2021Submitted to AIChE Journal
07 Jul 2021Submission Checks Completed
07 Jul 2021Assigned to Editor
11 Jul 2021Reviewer(s) Assigned
09 Aug 2021Editorial Decision: Revise Major
28 Aug 20211st Revision Received
31 Aug 2021Submission Checks Completed
31 Aug 2021Assigned to Editor
02 Sep 2021Reviewer(s) Assigned
21 Sep 2021Editorial Decision: Revise Minor
27 Sep 20212nd Revision Received
27 Sep 2021Submission Checks Completed
27 Sep 2021Assigned to Editor
29 Sep 2021Editorial Decision: Accept