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\begin{document}

\title{Snapshot of anti-SARS-CoV-2 IgG antibodies in Covid-19 recovered
patients in Guinea}



\author[1]{Solène GRAYO}%
\author[1]{Houlou Sagno}%
\author[2]{Oumar Diassy}%
\author[3]{Jean-Baptiste Zogbelemou}%
\author[4]{Sia Jeanne Kondabo}%
\author[3]{Marilyn Houndekon}%
\author[5]{Koussay Dellagi}%
\author[6]{Inès Vigan-Womans}%
\author[7]{Samia Rourou}%
\author[7]{Wafa Ben Hamouda}%
\author[7]{Chaouki Benabdessalem}%
\author[7]{Melika Ben Ahmed}%
\author[1]{Noel  Tordo}%
\affil[1]{Institut Pasteur de Guinée}%
\affil[2]{Agence Nationale de Sécurité Sanitaire}%
\affil[3]{Centre médico-social de l’ambassade de France}%
\affil[4]{Clinique Ambroise Paré}%
\affil[5]{Institut Pasteur}%
\affil[6]{Institut Pasteur de Dakar}%
\affil[7]{Institut Pasteur de Tunis}%


\vspace{-1em}



  
  \date{June 11, 2024}


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\begin{abstract}
Naturally-acquired immunity following primary SARS-CoV-2 infection was
investigated from 200 patients (90\% of African extraction) who
recovered from Covid-19 since at least \textasciitilde{} 2.4 months (72
days). The detection of IgG antibodies to SARS-CoV-2 were performed by
using an in-house ELISA assay against the Receptor Binding Domain (RBD)
of SARS-CoV-2 spike1 protein (RBD/S1-IH kit), showing 73\% of positive
sera (146/200) with an Optical Density (OD) ranging from 0.13 to 1.19
and a median value of 0.56 (IC95: 0.51-0.61). Median OD value at 3
months (1.040) suddenly decreased after and remains stable around OD 0.5
until 15 months post-infection. No significant difference was found
between male (M) and female (F) (median OD values :0.62 (M) vs 0.49 (F),
P-value:0.073). In contrast, the OD median value was significantly
higher among the 60-100 age group (0.87) compared to other groups, with
a noteworthy odds ratio compared to 0-20 age group (OR: 9.69, P-value:
0.044*). Compared to a nucleoprotein ELISA commercial kit, a better
concordance was found between anti-spike1 protein ELISA (RBD/S1-IH Elisa
kit and a whole spike1 protein ELISA commercial kit) and revealed also
higher seropositivity rates. These findings emphasize the relevance of
specific ELISA kits for accurate seropositivity rates.%
\end{abstract}%



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