Conclusion
This study demonstrated that Anopheles  mosquito species recognition may be accomplished with success using molecular characterization. DNA barcoding results, which utilized the genetic marker 18S rDNA, were equivalent to the morphological identification of some species and also allowed for identification  of morphologically misidentified species. It is crucial to highlight that in situations when conventional morphological classification was unsuccessful owing to damaged specimens and unidentifiable characteristics, DNA barcoding successfully identified these species. The newly discovered sequence could potentially be utilized as reference sequences in future mosquito taxonomy investigations. The successful identification of numerous mosquito species in this study will assist with malaria research by determining the real and prospective danger of arbovirus or parasite transmission, as well as providing fundamental knowledge for vector surveillance and enhancing vector control strategies. In the future, molecular identification implementations should include barcoding a greater number of species and incorporating other genetic markers that improve the discriminatory strength of this identification approach. DNA barcoding might also be used in conjunction with next generation sequencing to identify huge numbers of mosquitos at once, considerably reducing the processing time required for species characterization.