Diversity Index Analysis of Phyllosphere Bacteria of Rubber
Trees with Powdery Mildew
The member abundance and diversity of the phyllosphere microbiome
community were studied by analyzing the diversity of individual samples
(alpha diversity) using various statistical indexes (Table S3).
The OTU statistics were analyzed at a 97% similarity level, and the OTU
numbers for the 16 samples ranged from 24 to 324. Using the Ace index
and Chao index, the total number of species in the phyllosphere
microbiome was estimated to be 34–329 and 29–329, respectively. The
Shannon index and Simpson index estimated samples’ microbial diversity
at 0.14–5.09 and 0.01–0.96, respectively. The coverage of the 16
samples exceeded 99%, confirming that the sequencing results accurately
represented the microorganisms in rubber tree leaves. Despite their
distinct algorithms, the Ace index and Chao index generally yielded
consistent results and did not differ considerably in terms of measured
OTU numbers. Both the Shannon index and Simpson index revealed a ranking
of WZS > WN > DZ > BS for the
alpha diversity in the microbiota of the four regions.