Fish parvalbumins and extracts
The parvalbumins (PV), raw extracts (RE) and heated extracts (HE) used in the ALEX2 assay are listed in Table SII . PV purity and protein composition of the extracts are visualized inFigure S3 . The methods used for PV and extract preparation are provided in the supplementary material. The phylogenetic analysis of PV sequences was performed in Jalview 2.11.1.3.15 The sequences were aligned using ClustalO and the phylogenetic tree computed using the neighbor-joining algorithm.