Glaucopysche alexis annotation
Prior to annotation, a de novo repeat library was made using RepeatModeller v2.0.1 (27) and was then provided to RepeatMasker v4.1.1 to detect and mask repetitive sequences (28). Protein-coding genes and transcripts were predicted using BRAKER2 v2.0.6 (29), using protein hints which were generated using the ProtHint protein mapping pipeline v2.6 (30) with the reference protein sequences for arthropods from OrthoDB (31) as homology evidence.