1.4 Laboratory examinations
Total nucleic acids were extracted and tested for the respiratory syncytial virus. Other eight respiratory viruses (influenza virus A and B [FLU A, FLU B], influenza virus AH1N1 2009 pandemic and AH3N2 [AH1N1 2009, AH3N2], parainfluenza virus 1, 2, 3, 4 [PIV 1, 2, 3, 4], adenovirus [AdV], human rhinovirus [HRV], human metapneumovirus [HMPV], human coronavirus 229E/NL63, OC43/HKU1 [CoV 229E/NL63, OC43/HKU1], human bocavirus [HBoV], and human enterovirus [EV]), M. pneumoniae (MP), andChlamydophila pneumoniae (CP) were also identified according to the design of this study using a real-time PCR-based approach (Multiplex Combined Real-time PCR Detection Kit for Respiratory Viruses, Jiangsu Uninovo Biological Technology Co. Ltd., China). RSV-positive samples were further identified into RSV group A (RSV-A) and RSV group B (RSV-B) (Duplex Real-Time PCR Kit for RSV, Beijing Kinghawk Pharmaceutical Co., Ltd., China).