ELISA
Plasma IL-15, sMAdCAM-1 and LPS levels were quantified using Human IL-15
DuoSet ELISA kit (R&D Systems), Human MAdCAM-1 DuoSet ELISA kit (R&D
Systems) and Human Lipopolysaccharides ELISA kit (MyBioSource)
respectively, following manufacturers’ recommendations. The values below
the level of detection were assigned as zero.