Figure 1. Distribution of CD4 and CD8 T subsets in the blood.

(A) Representative flow cytometric analysis of the gating strategy, leukocytes (CD45+), T cell (CD3+) and CD4 and CD8 T cells. (B) Based on CD45RA and CCR7 expression, CD4 and CD8 have 4 main subsets; naïve (CD45RA+CCR7+), central memory (TCM, CD45RA-CCR7+), effector memory (TEM, CD45RA-CCR7-), revertant effector memory TEMRA (CD45RA+CCR7-). CD4+ subset in the upper panel and CD8+subsets in the lower panel. (C) The four main subsets (Naïve (dark blue), TCM (green), TEM (light blue) and TEMRA (orange) are further divided into a different subset based on surface expression of CD27 and CD28. (D) CD4 is separated into T follicular helper cells (PD-1+CD45RA-). CD4 and CD8 are divided into cytotoxic (PD-1+CD57+) and senescence cells (PD-1-CD57+). CD4+ subset in the upper panel and CD8+subsets in the lower panel.