Quantity of Bet v 1 (Q) inducing a conjunctival response
As the allergen affected the ocular surface, we used the tear film
renewal rate (TRR) to estimate the amount of extract applied during EEC
exposure. The TRR was calculated to be 154×106mm3/min by Beaudouin et al.18 Thus,
the quantity (Q) of extract applied was calculated in nanograms as
follows: Q = C x Time x TRR, where C is the concentration of airborne
Bet v 1 in ng/m3 and Time is the time required to
induce the conjunctival response in minutes.