Single-cell transcriptional profiles of COVID-19 patients
Single-cell sequencing data of eight healthy lung tissues, three mild,
and six severe lung lavage fluid from COVID-19 patients were downloaded
from the GEO database with the accession number GSE145926 (17). The
quality control, standardization, integration, dimensionality reduction,
and unsupervised cell clustering of
the
samples were carried out using R package Seurat (v3.0.2). For each
patient, the same standard for quality control was used: 1) each cell
expressed 200-6,000 genes, and the number of expressed genes was greater
than 1,000; 2) each gene was expressed in at least three cells; 3) The
proportion of mitochondrial genes was less than 10%.