Figure 2: (A) Cell viability in sheet-like cocultured substitutes after one month. Live/Dead fluorescence staining of MSCs and ECs cocultured on BCP granules. Calcein AM in green (living cells) and EthD-1 in red (dead nuclei). (B) Cell organisation in sheet-like substitutes after one-month culture. Actin filament and Cx-43 staining of MSCs and ECs cocultured on BCP granules. Rhodamine-phalloidin in red (actin filaments) and Cx-43 immunofluorescence in green (gap junctions). (C) Cell morphology in sheet-like substitutes after one-month culture observed with Scanning electron microscopy. Donors 1 to 6; 7: MSC; 8: MSC diff.