Figure 2: (A) Cell viability in sheet-like cocultured
substitutes after one month. Live/Dead fluorescence staining of MSCs and
ECs cocultured on BCP granules. Calcein AM in green (living cells) and
EthD-1 in red (dead nuclei). (B) Cell organisation in sheet-like
substitutes after one-month culture. Actin filament and Cx-43 staining
of MSCs and ECs cocultured on BCP granules. Rhodamine-phalloidin in red
(actin filaments) and Cx-43 immunofluorescence in green (gap junctions).
(C) Cell morphology in sheet-like substitutes after one-month culture
observed with Scanning electron microscopy. Donors 1 to 6; 7: MSC; 8:
MSC diff.