Supplementary Figure 2
(A-B). Bar charts, with representative FACS gating, visualizing the proportions of cells expressing CD49b+(A) and FCER1A+ (B) from each cluster identified by Seurat.
C. Bar chart comparing the proportions of the clusters identified by Seurat (blue) and Spectre (red) within the total CD8+ T cell compartment. Clusters uniquely identified by one method are circled.
D. Uniform manifold approximation and projection (UMAP) plots visualizing the clustering results from Seurat (left) and Spectre (right). Cells that are differently clustered by Seurat and Spectre are circled. Sp2 from Spectre (i) is divided into Se1, Se2 and Se8 by Seurat (I). Se4 from Seurat (II) is split into Sp1 and Sp4 by Spectre (ii).
N=5 per group and data are presented as mean ± s.e.m.