Supplementary Figure 2
(A-B). Bar charts, with representative FACS gating, visualizing
the proportions of cells expressing CD49b+(A) and FCER1A+ (B) from each
cluster identified by Seurat.
C. Bar chart comparing the proportions of the clusters
identified by Seurat (blue) and Spectre (red) within the total
CD8+ T cell compartment. Clusters uniquely identified
by one method are circled.
D. Uniform manifold approximation and projection (UMAP) plots
visualizing the clustering results from Seurat (left) and Spectre
(right). Cells that are differently clustered by Seurat and Spectre are
circled. Sp2 from Spectre (i) is divided into Se1, Se2 and Se8 by Seurat
(I). Se4 from Seurat (II) is split into Sp1 and Sp4 by Spectre (ii).
N=5 per group and data are presented as mean ± s.e.m.