Figure 2 Comparative analysis of T cell profiles in peripheral blood mononuclear cells (PBMCs) from adult blood (AB) and cord blood mononuclear cells (CBMCs) from cord blood (CB)
A. An overview of the adult blood (AB) and cord blood (CB) study design. Peripheral blood mononuclear cells (PBMCs) and cord blood mononuclear cells (CBMCs) were isolated and then were first labelled with anti-CD45 antibodies with different fluorophores or their combinations for barcoding. After that, PBMCs were pooled together and stained with our 20-marker antibody panel and analyzed with Spectral Cytometry. Next, the resulting data were first demultiplexed based on their CD45 marker signals and then subject to analysis with Seurat and manual gating.
B. Representative flow cytometric plots of CD4+ and CD8+ T cells from AB and CB to identify naïve (CD45RA+CD27+), central memory (CM, CD45RA-CD27+), effector memory (EM, CD45RA-CD27-) and effector memory cells re-expressing CD45RA (EMRA, CD45RA+CD27-) subsets.
(C-D ) Scatter bar charts for the proportions of naïve, CM, EM, and EMRA subsets within CD4+ (C ) and CD8+ (D ) T cells from AB and CB.
(E-F ) Bar charts for the log2(fold change) comparing AB versus CB for the proportions of populations expressing the corresponding markers among CD4+ (E) and CD8+ (F) T cells. The asterisks denote the populations whose proportions are significantly different between AB and CB.
N=5 per group and data are presented as mean, with * p<0.05 and ** p<0.01 by unpaired Mann-Whitney U test.