Supplementary Figure 8
A. Uniform manifold approximation and projection (UMAP) plots
visualizing the clustering results from Seurat (left) and Spectre
(right) based on the adult peripheral blood mononuclear cells (PBMCs)
CD4+ T cell experiment. One color represents one
cluster.
B. Dot plots visualizing the clusters identified by Seurat
(left) and Spectre (right) and their marker expression profiles. The
size of the dot corresponds to the percentage of cells expressing the
corresponding markers and the color gradient reflects the average
normalized expression of the corresponding markers.
C. Venn diagram comparing the clustering results from Seurat
and Spectre. Both methods were set to generate 15 clusters and 12 out of
15 clusters could be identified by both methods, while Se2, Se8, and
Se15 could only be identified by Seurat and Sp1, Sp8 and Sp15 could only
be identified by Spectre.
D. Scattering dot plot comparing the proportions per sample
within the total CD4+ T cell compartments of the
clusters identified by Seurat or retrieved by manual gating.
N=5 per group and data are presented as mean ± s.e.m.