Figure 4 Seurat analysis identified a unique CD8+CD45RA+CD27+CD161+T cell population in cord blood
A. Overlay of the newly identified CD8+CD45RA+CD27+CD161+T cell subset (cluster 8) onto the Uniform manifold approximation and projection (UMAP) plots for AB (left) and CB (right) CD8+ T cell compartments.
B. Manual gating strategies to identify the CD8+CD45RA+CD27+CD161+T cell subset (cluster 8) from AB (left) and CB (right) CD8+ T cell compartments.
C. Scatter bar chart for the proportions of CD8+CD45RA+CD27+CD161+T cell subset (cluster 8) in AB (black) and CB (yellow) CD8+ T cell compartments.
D. Scatter dot plot of the proportions of cells expression integrin β7 in CD8+CD45RA+CD27+CD161+and CD8+CD45RA+CD27+CD161-T cell subsets.
E. Bar chart for the log2(fold change) comparing the proportions of populations expressing the corresponding markers among CD8+CD45RA+CD27+CD161+and CD8+CD45RA+CD27+CD161-T cell subsets. The asterisks denote the populations whose proportions are significantly different between AB and CB.
N=5 per group and data are presented as mean, with * p<0.05 and ** p<0.01 by unpaired Mann-Whitney U test.
F. Scatter dot plot of the proportions of cells expressing IFN-γ, IL-4, IL-5, IL-10, and IL-13 in CD8+CD45RA+CD27+CD161+and CD8+CD45RA+CD27+CD161-T cell subsets.