Figure 3 Unbiased comparative analysis of CD8+T cell profiles in peripheral blood mononuclear cells (PBMCs) from adult blood (AB) and cord blood mononuclear cells (CBMCs) from cord blood (CB) with principal component analysis (PCA) and Seurat
A. PCA based on the marker expression levels on CD8+ T cells from AB and CB samples.
B. Uniform manifold approximation and projection (UMAP) plot visualizing the clustering results from Seurat and the compositional contribution of each cluster from AB versus CB. The donut charts visualized the proportions of each cluster that are from AB (dark blue) and CB (yellow), and the numbers within denoted the proportions of the corresponding clusters within the overall CD8+ T cells from both AB and CB.
C. Dot plot visualizing the clusters identified by Seurat and their marker expression profiles. The size of the dot corresponds to the percentage of cells expressing the corresponding markers and the color gradient reflects the average normalized expression of the corresponding markers.
D. Projection of 13 clusters identified by Seurat based on the AB versus CB experiment onto the two-dimensional (2D) plot comparing their expression of CD27 and CD45RA. The dashed lines denote the average normalized expression of CD27 and CD45RA for all cells.
E. Dot plot comparing the clusters from AB versus CB identified by Seurat and their marker expression profiles. The size of the dot corresponds to the percentage of cells expressing the corresponding markers and the color gradient reflects the average normalized expression of the corresponding markers.