Supplementary Figure 9
(A-B ). Scatter dot plots of the proportions of CD4+ (A ) and CD8+(B ) T cells expressing different surface and intracellular markers in adult blood (AB) versus cord blood (CB).
(C-D). Scatter dot plots of the proportions of CD4+ (C ) and CD8+(D ) T cells expressing IFN-γ, IL-4, IL-5, IL-10, and IL-13 in adult blood (AB) versus cord blood (CB).
(E-F ). Clustering results retrieved by Spectre for CD8+ T cells based on the combined AB and CB experiment. E. Uniform manifold approximation and projection (UMAP) plot visualizing clustering results from Spectre. F. Dot plot visualizing the clusters identified by Spectre and their marker expression profiles. The size of the dot corresponds to the percentage of cells expressing the corresponding markers and the color gradient reflects the average normalized expression of the corresponding markers.
N=5 per group and data are presented as mean ± s.e.m., with * p<0.05 and ** p<0.01 by unpaired Mann-Whitney U test.