Figure 3 Unbiased comparative analysis of CD8+T cell profiles in peripheral blood mononuclear cells (PBMCs) from adult
blood (AB) and cord blood mononuclear cells (CBMCs) from cord blood (CB)
with principal component analysis (PCA) and Seurat
A. PCA based on the marker expression levels on
CD8+ T cells from AB and CB samples.
B. Uniform manifold approximation and projection (UMAP) plot
visualizing the clustering results from Seurat and the compositional
contribution of each cluster from AB versus CB. The donut charts
visualized the proportions of each cluster that are from AB (dark blue)
and CB (yellow), and the numbers within denoted the proportions of the
corresponding clusters within the overall CD8+ T cells
from both AB and CB.
C. Dot plot visualizing the clusters identified by Seurat and
their marker expression profiles. The size of the dot corresponds to the
percentage of cells expressing the corresponding markers and the color
gradient reflects the average normalized expression of the corresponding
markers.
D. Projection of 13 clusters identified by Seurat based on the
AB versus CB experiment onto the two-dimensional (2D) plot comparing
their expression of CD27 and CD45RA. The dashed lines denote the average
normalized expression of CD27 and CD45RA for all cells.
E. Dot plot comparing the clusters from AB versus CB identified
by Seurat and their marker expression profiles. The size of the dot
corresponds to the percentage of cells expressing the corresponding
markers and the color gradient reflects the average normalized
expression of the corresponding markers.