Isolation of protozoan and vitality identification
Protoscocras(PSCs) were obtained from E. multilocularis-infected gerbils. In briefly, E.multilocularis infected cyst tissue were isolated from gerbils and stripped components of mouse. PSCs were isolated immediately by pushing the cyst through a 300-mesh strainer in PBS buffer, and collected filtrate. The filtrate was naturaly settlemented for 5 minutes, discard the supernatant and wash 2 times with PBS. PSCs were stained with 1% eosin (Beyotime, China) to test vitality. If the vitality was over 95%, the PSCs could inject in mice.