Target prediction of the differential miRNAs
Differential miRNAs target gene prediction was performed using both
software A and B and target genes predicted by both software were
screened according to scoring criteria. target genes with context score
percentage less than 50 are removed in targetscan algorithm. Target
genes with a maximum free energy (Max Energy) greater than -10 are
removed in miRanda algorithm (i.e. the threshold is TargetScan score ≥
50, miranda_Energy < - 10). Finally the intersection of these
two software was taken as the final target gene of the differential
miRNAs.