Methods
CD4+ T cells of patients with birch pollen and
associated hazelnut allergies were differentiated into Bet-specific or
non-specific induced Treg (iTreg). After Bet- or Cor- specific
restimulation the phenotype, proliferation and suppressive capacity of
iTreg subsets were analyzed. iTreg function was further investigated in
humanized mouse models of airway and intestinal allergy, generated by
engraftment of peripheral blood mononuclear cells from allergic donors
into immunodeficient animals.