Humanized mouse model of type I allergy
As described previously40,41,
NOD.CB17-Prkdcscid/Jγc-/- mice were
engrafted with PBMC from donors with birch pollen and associated
hazelnut allergy and were simultaneously injected with birch pollen
extract (=PBMC + birch, allergic positive control) or without (=PBMC,
negative control). iTregBet were co-injected at day 0
into animals treated like the allergic positive control. After three
weeks, blood samples for human IgE analysis were taken, the mice were
challenged rectally with birch pollen or hazelnut extract and the
allergen-induced intestinal inflammation was scored endoscopically. On
the next day the mice were challenged intranasally with birch pollen
extract and allergen-induced airway inflammation was evaluated by
measurement of methacholine (MCh)-provoked airway resistance.