Induction of iTreg and effector T cells (Teff)
Immature dendritic cells (DC) from donors with birch pollen and hazelnut
allergies were generated by culture of plastic-adherent peripheral blood
mononuclear cells (PBMC) with IL-4 and GM-CSF for 6 days and were then
differentiated into mature DC (mDC) by addition of a maturation cocktail
or into IL-10 DC by addition of the maturation cocktail and IL-10
(Supplementary Figure 1). Bet-loaded mDC (mDCBet) or IL-10 DC (IL-10
DCBet) were generated by addition of rBet v 1.0101 during DC
differentiation. Autologous CD4+ T cells were
cocultured with mDC or IL-10 DC to prime effector T cells (Teff) or
iTreg, respectively.