Suppressor assay (SA)
CD4+CD25low responder T cells (Tresp) from donors with birch pollen and associated hazelnut allergy were stimulated with autologous Bet- or Cor- loaded mDC (control, ctrl). iTreg primed by IL-10 DCBet (iTregBet) or IL-10 DC0 (iTreg0) were added in 1:1 or 1:2 Tresp:iTreg ratios (SA 1:1 / SA 1:2), respectively. Tresp, mDC and iTreg were stained with different cell proliferation dyes for identification and assessment of T cell proliferation.