Question 2: The novelty is not found in this paper. There have
been many reports on the optimization of conditions for DAG, and the
comparison of C-oil, C-FFA, and C-DAG has no research significance.
Thanks for your helpful comments. The novelty for this paper lies in the
fact that the higher content of diglycerides is obtained by a two-step
enzymatic coupling, which simplifies the purification process that needs
to be carried out in order to obtain a high content of diglycerides, and
achieves the diglyceride content that most of the current reports need
to be purified in order to achieve, and reduces the cost problems
associated with the purification step. The abstract of the article has
been revised to highlight the significance of the methodology by
replacing the abstract on page 2, lines 16-19 in the original manuscript
with the following:
The results showed that under the optimal conditions of hydrolysis and
esterification, the hydrolysis rate was 87.14% and the DAG content was
62.49%. The high content of DAG was obtained by the coupling method,
which reduced the cost of purification step in the production process.