Question 2: The novelty is not found in this paper. There have been many reports on the optimization of conditions for DAG, and the comparison of C-oil, C-FFA, and C-DAG has no research significance.
Thanks for your helpful comments. The novelty for this paper lies in the fact that the higher content of diglycerides is obtained by a two-step enzymatic coupling, which simplifies the purification process that needs to be carried out in order to obtain a high content of diglycerides, and achieves the diglyceride content that most of the current reports need to be purified in order to achieve, and reduces the cost problems associated with the purification step. The abstract of the article has been revised to highlight the significance of the methodology by replacing the abstract on page 2, lines 16-19 in the original manuscript with the following:
The results showed that under the optimal conditions of hydrolysis and esterification, the hydrolysis rate was 87.14% and the DAG content was 62.49%. The high content of DAG was obtained by the coupling method, which reduced the cost of purification step in the production process.