Supplemental Figures
FIGURE S1 Abdominal ultrasonography performed on admission (10 days after birth). Size of cystic lesion is indicated.
FIGURE S2 Abdominal computed tomography images performed 5 days after birth. Arrow indicates a small linear calcification in the middle of the solid part.
FIGURE S3 Immunohistochemical findings of osteoblast-like cells (left panels) and spindle cells (right panels) stained by anti-WT1 (top panels) and anti-epithelial membrane antigen (EMA) antibodies.
FIGURE S4 Whole-exome sequencing (WES) was performed on the tumor sample and her peripheral blood mononuclear cells (PBMC). The genomic DNA was sequenced on Illumina NovaSeq6000. WES was performed using the QIAGEN CLC Genomics Workbench 23.0.4 software. Golden Helix VarSeq 2.3.0 was used for data assessment. Tumor-specific missense mutation of CTNNB1 (c.121A>G, p.T41A) was identified with 0.38 of variant allele frequency (VAF). Genomic sequences of PBMC (top) and tumor sample (bottom) are aligned.
FIGURE S5 Sanger sequencing of the TERT promoter was performed using genomic PCR products of the patient’s tumor sample and her peripheral blood mononuclear cells (PBMC). Arrows indicate -146C and -124C, which are reportedly mutated to T in all sequenced cases of calcifying nested stromal-epithelial tumor (CNSET) 14.