ELISA
Levels of inflammatory cytokines in BALF supernatants were detected using IL-1β (MLB00C, R & amp, USA; D Systems, Minneapolis, USA) and IL-6 (M6000B, R & amp; D Systems, USA) enzyme-linked immunosorbent assay (ELISA) kits. Experimental procedures were performed according to the manual. Information of ELISA kits can be found in Supplementary Table 2.