TAG and PLs Analysis
The lipid content in KO emulsions was determined using the MK-6S TLC-FID Analyzer (Iatroscan, Japan). Lipids were extracted using a chloroform-methanol mixture and dissolved in chloroform at concentrations ranging from 10 to 20 mg/mL. Separation was achieved using n-heptane, anhydrous ether, and formic acid (in a ratio of 42:28:0.3) as the developing agent. The analysis involved specific conditions such as air and hydrogen flow rates, as well as scanning speed, following the method outlined by Fu et al. (2023).