Particle Size and Zeta-Potential Analysis
Particle size distribution and zeta-potential measurements were
conducted using a Laser particle size analyzer (Zetasizer 2000, Malvern
Instruments, Worcestershire, UK). Prior to analysis, emulsions were
diluted 20 times with deionized water. The measurements were performed
at a constant temperature of 25 ℃, with a fixed detection angle of 90°.
Each measurement was replicated using a minimum of three freshly
prepared samples (Francisco et al., 2023).