PC Analysis
The quantification of PC content was carried out using HPLC with a Shimadzu LC-20AD Prominence HPLC system equipped with an Alltech ELSD 6000 detector. The analysis included precise control of gas flow rate and ELSD tube temperature. Separation was achieved using an Agilent Zorbax RX-SIL column, employing a specific mobile phase composition. PC content was calculated based on standard curve methods, using GPCho (C16:0/18:1) and GPEtn (C16:0/18:1) standards (Li et al., 2020).