DHA and EPA Analysis
The fatty acid profiles of KO emulsions, with a specific focus on
changes in EPA and DHA during accelerated storage, were determined using
GC-MS (Agilent, America). This process involved the removal of
unsaponifiable matter, extraction of saponifiable matter with hexane,
and analysis under controlled conditions, including temperature
gradients and injection volume, following the method described by Li et
al. (2019).