MtDNA genotyping and analyses
The mitochondrial cytb gene was sequenced following the PCR protocols in Hammer et al. (2014), except that samples were amplified with the following primer pairs: Glu18 TAACCAGGACTAATGRCTTGAA with Hd.alt GGRTTGTTGGAGCCTGTTTCAT or Hd.Hyps GGGTTGTTGGAGCCSGTTTCGT and midg.496 GGCGGCTTTTCRGTAGATAA with Eleo.Thr.40 GATTTTAACCTCCTGCGTCCG. Sequences were edited using Chromas 2.6.5 (Technelysium, Tewantin, Queensland, Australia) and imported into BioEdit 7.2.5 (Hall 1999). Sequences were aligned by eye and checked via amino acid coding in MEGA to test for unexpected frame shift errors or stop codons. Data were analysed phylogenetically using RAxML as per for the SNP analysis but using the model GTRGAMMA.