Plasmids
Plasmids were amplified using E.Coli DH5α strain (Life Technologies). DNA was then purified using the NucleoBond® Xtra Plus Midi-Prep kit (Machery-Nagel®). The plasmids used were: pcDNA3.1-rGFP-CAAX , pcDNA3.1-p63RhoGEF-RlucII , pcDNA3.1-Gαq (gift from Jean-Philippe Pin), pRK5-SNAPTag-AT1 (CIS bio international), pRK5-Myc-mGlu5a and pcDNA3.1-Myc-mGlu5a-Venus . pCAGGS-TRPC6-ires2-mbtdtomato was obtained by cloning mouse TRPC6 sequence in pCAGGS plasmid upstream the sequences of IRES2 and membrane-anchored tdtomato.