Author contributions
MB and JP conceived research and wrote the manuscript; MB carried out the majority of the experiments, conception, design of the protocols, analysis of the results and production of the figures. CC carried out G protein sensor experiments, including protocol design, experiments and analyses. NB performed theoretical and manual supervision of the molecular biology experiments (electrophoresis, plasmid digestion, transfection) and data interpretation. YC provided general assistance with the acquisition and analysis of results in microscopy, he also developed optimised programmes to facilitate and automate signal analysis. JFI helped to design and perform experiments to detect mGlu5 receptor conformation. EM supervised and implemented neuronal cultures with NB and the development of population BRET protocols. LT cloned the pCAGGS-TRPC6-ires2-mbtdtomato plasmid. JC took an active part in the implementation of the experimental protocol and together with LT provided advices for patch clamp recordings on HEK and analysis of results. JP carried out BRET and calcium microscopy experiments in neurons, and was involved in the analysis of results and theoretical supervision at all levels. JP conceived and supervised the full project. All authors contribute to the preparation of the manuscript and approved it.