Reagents
(S)-3,5- Dihydroxyphenylglycine (DHPG, HelloBio®); Quisqualate (Tocris); LY341495 (Tocris®); 2-methyl-6-(phenylethynyl)-pyridine hydrochloride (MPEP, HelloBio®); human angiotensin II (HelloBio®); N-Methyl-D-Aspartate (NMDA, Tocris®). 7-(Hydroxyimino)cyclopropa[b ]chromen-1a-carboxylate ethyl ester (CPCCOEt, Tocris®); Tetrodotoxin (TTX) Citrate (Latoxan®), Bicuculline (Hellobio®). Except for dose response curves, all the mGlu5, AT1 and mGlu1 ligands are applied at saturating concentrations.