Determination of frequency-dependent filtering in the LPP – DG synapse
Synaptic frequency-dependent filtering was examined in the presence of DCG-IV (5 µM), and a baseline response of fEPSP slope configured at 50% of its maximal amplitude was acquired for 10-12 min. Then, a train of 10 current pulses (100 µs of duration) at 5, 20, and 50 Hz was delivered to the LPP, with an interval of 10 min between trains, as previously reported (Quintanilla et al., 2022). For each stimulation frequency, the train’s evoked synaptic responses were normalized to the slope of its first synaptic response and plotted as the number of stimuli vs. normalized fEPSP slope (%).