2.2 Construction of plasmids and recombinant strains
All plasmids used in this study are listed in Table 1 and the primers
are listed in Table S1. The first is the linearization of the vector
pMV261 and the amplification of the target genes. The co-expression
genes hsd4A -kshA1 and kshA1 -hsd4A were
constructed and amplified by overlap PCR. Then the genes of interest
obtained by PCR amplification were inserted into pMV261 through the
in-fusion clone method to construct the recombinant plasmids. The
recombinant plasmids were transferred into HK86 W via electroporation to
construct recombinant Mycobacterium .