Erythrocytes
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Small volume of blood (3-10\(\mu\)l) required so suitable for finger
prick test and multiple sampling.
Doesn’t require any blood pre-processing so less hands on time.
Reticulocyte percentage (%RET) in whole blood can be analysed
simultaneously as a measure of haematopoietic function.
Can detect an accumulation of DNA damage from repeat dosing over a
prolonged period; long term effect.
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Cannot confirm mutant phenotype by DNA sequencing.
Unsuitable for cryopreservation and batching samples from remote
locations.
PIG-A mutant erythrocytes may be subject to complement mediated
lysis.
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Reticulocytes
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May be able to observe mutant induction sooner post exposure than
erythrocytes.
Reticulocyte percentage (%RET) in whole blood can be analysed
simultaneously as a measure of haematopoietic function.
PIG-A mutant reticulocytes would not be sensitive to complement
mediated lysis.
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Proportion of reticulocytes is low in whole blood compared to
erythrocytes so would require larger blood volume.
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Granulocytes
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May be able to observe mutant induction sooner post exposure than
erythrocytes.
Allows for mutation confirmation using DNA sequencing.
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Requires blood pre-processing and a more complex flow cytometry gating
strategy.
Short life span of 1-2 days provides a small window of opportunity to
capture mutant cells.
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Peripheral blood mononuclear cells
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Allows for mutation confirmation using DNA sequencing.
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Published methodology includes subculture of PBMCs under Aerolysin
selection.
Requires larger volume of blood.
Current studies show higher background level of mutant cells compared to
erythrocytes.
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