a ) All enzymes were purified before
characterization. For determining kinetic parameters, the initial rate
of the enzyme was measured in 100 mM Tris-HCl pH 8.0 with
NAD+ or NADP+ (0.2 mM) in the
presence of saturating concentrations of substrate galactitol (50 mM) at
30 °C; b ) i.g. = infinitely great;c ) n.a. = no measurable activity.
Table 3. Information on the pathway conditions.