Application of Shear Forces
This study uses the cone and plate bioreactors (CPB) to investigate the fluid shear stress experienced by endothelial cells (EECs) in the left ventricular outflow tract (LVOT) of patients with DSS. The CPB system operates based on the following principle: an inverted cone is positioned above a stationary flat plate, creating a narrow gap through which fluid flows. This arrangement ensures the distribution of uniform shear stress across the surface [46].
To control the fluid flow, a magnetic stir plate is employed, which enables adjustment of the cone’s angular velocity by modifying the revolutions per minute (RPM) setting on the control unit. This mechanism allows for precise manipulation of the shear stress experienced by the EECs in the CPB system [46].