3.2. Multi-generational effects on lipid metabolism
The multigenerational effects of DEHTP on the lipid metabolites,
including TG, NEFA, ACA and FA-CoA, are shown in
Figure S2. In F1, at 0.8 mg/L
DEHTP inhibited TG, ACA, lipase, ATGL, ACS, CPT and ACC but stimulated
NEFA, FA-CoA, GPAT and FAS. In F2 to F4, the effects showed alterations
between inhibition and stimulation without specific tendencies or
relationships (Figure S2).
Notably, there were opposite effects on TG and NEFA, with 60% of the
cases where the stimulations on TG were usually accompanied with
inhibitions on NEFA and vice versa (Figure 3(A)). The opposite effects
were also found between lipase and ATGL (40% of the total cases), and
also between the effects on ATGL and GPAT (55% of the total cases)
(Figure 3(B)).
The Spearman’s correlation analysis demonstrated the connection between
the metabolites and the enzymes (Figure 3(C)). The significant
stimulations of DEHTP on ACA were accompanied with positively correlated
stimulation on a series of enzymes in ACA metabolism (ACS, CPT and ACC)
and also in the balance between TG and NEFA (ATGL and GPAT). Combining
the apical and biochemical effects, there were positive correlations
between the effects on reproduction and with those on FAS and FA-CoA,
and negative ones between the influences on behavior and those on
FA-CoA, ACA and GPAT (Figure 3(E)).