4.4. Combined analysis on the multi-generational influences of
DEHTP
Neural signals not only regulate behavior, but also connect with lipid
metabolism and reproduction (Luo et al., 2022; Magnan et al., 2015). The
positive correlations between biochemicals in the neural regulation and
those in lipid metabolism supports the neural regulation on lipid
metabolism (Ali and Mirza, 2021; Crane et al., 2015). The oscillatory
changes in the HCA results demonstrated the active responses of
organisms to the multi-generational signals. Such multigenerational
responses can involve epigenetic regulations (e.g., DNA methylations),
which were observed in the disturbances of DEHP on liver in mice over
three generations (Wen et al., 2020). Notably, the 5-HT results in HCA
demonstrated an oscillatory pattern over generations, which could be
related with the oscillation between stimulatory and inhibitory apical
effects of DEHTP over generations. Future studies should consider both
transcriptomic and epigenomic analysis to further explore mechanism.