5. Conclusion
In summary, DEHTP showed a regular oscillation pattern in the
multigenerational reproductive toxicities, with stimulation in F1 and F3
generations and inhibition in F2 and F4 ones. The influences of DEHTP on
reproduction and lifespan showed trade-off relationships. Further
analysis demonstrated the involvement of lipid metabolism and neural
regulation underlying the multi-generational effects. HCA results
pointed out the oscillatory connection between 5-HT and other neural
regulation in F1 and F3 and between 5-HT and other indicators in F2 and
F4. The overall oscillatory effects of DEHTP over generations need
further transcriptomic and epigenomic analysis to explore the underlying
mechanisms.