4.4. Combined analysis on the multi-generational influences of DEHTP
Neural signals not only regulate behavior, but also connect with lipid metabolism and reproduction (Luo et al., 2022; Magnan et al., 2015). The positive correlations between biochemicals in the neural regulation and those in lipid metabolism supports the neural regulation on lipid metabolism (Ali and Mirza, 2021; Crane et al., 2015). The oscillatory changes in the HCA results demonstrated the active responses of organisms to the multi-generational signals. Such multigenerational responses can involve epigenetic regulations (e.g., DNA methylations), which were observed in the disturbances of DEHP on liver in mice over three generations (Wen et al., 2020). Notably, the 5-HT results in HCA demonstrated an oscillatory pattern over generations, which could be related with the oscillation between stimulatory and inhibitory apical effects of DEHTP over generations. Future studies should consider both transcriptomic and epigenomic analysis to further explore mechanism.