3.3. Multi-generational effects on neural regulation
In F1, at 0.8 mg/L DEHTP stimulated 5-HT, DA, AChE and GABA and inhibited ACH, with down-regulations on the expressions of ser-1 ,ser-6 , mod-1 and dop-3 (Figure S3). In F2 to F4, DEHTP stimulated 5-HT, DA, AChE, ACH, GABA, with both up- and down-regulations on the gene expressions. Moreover, DEHTP caused mostly stimulations of 5-HT, DA, AChE and GABA, while it provoked mostly inhibition of ACH. The results showed the opposite effects on AChE and ACH, where stimulations on AChE with inhibition on ACH (and vice versa) covered 55% of the total cases (Figure 4). Moreover, the effects of DEHTP on 5-HT and DA showed positive Spearman’s correlation with each other (0.5534, p < 0.05). However, there were no correlations between the effects on neural regulations and those on locomotion (Figure 4).