2.2. Nematode preparation
Escherichia coli (E. coli ) OP50 was cultured in lysogeny
broth (LB) medium at 37 ºC and served as the food for subsequent
nematode culture. Caenorhabditis elegans (C. elegans ,
wild-type N2) nematodes were cultured at 20 ºC on nematode growth medium
(NGM) agar. Gravid hermaphrodite nematodes were lyzed by fresh Clorox
solution (1% NaClO and 0.5 mol/L NaOH), and the eggs were resistant to
such lysis and therefore were age-synchronized for subsequent
experiments (Li et al., 2019).