Discussion
In this study, we successfully
developed a generic pump-free organ-on-a-chip platform and used it to
mimic the physiological characteristics of the human intestinal barrier.
After determining the appropriate flow rate through fluid simulation,
the intestinal cells cultured on our platform proliferate and
differentiate vigorously, forming an intestinal barrier and
demonstrating its function through the oscillation of the rocker to
provide fluid. Subsequently, we investigated the impact of fluid
dynamics on cells in our system using RNA-seq analysis, which revealed
significant differences in metabolic pathways. Based on these promising
results, we proceeded to evaluate the bioavailability of drugs using our
gut-on-a-chip model, achieving excellent linear relationship
simulations. These data conclusively confirm the applicability of our
platform and its potential as a substitute for traditional organ chip
models.
Looking ahead, our chip platform has the capability to simulate various
human physiological systems, particularly those at the interface in
vivo, such as the blood-brain barrier, alveoli, glomeruli, and more. By
enabling the culture of different types of cells on both sides of the
membrane and providing shear force through a rocker mechanism to
simulate different body fluid conditions, we can further enhance the
versatility of our platform. However, we recognize the need to address
specific limitations and challenges to maximize the potential of our
chip system. This includes enhancing the longevity and stability of
cultured cells, optimizing fluidic dynamics within the chip, and
expanding the range of cell types to better emulate the complexity of
the human body.
In the future, we hope to establish
a powerful model that integrate organ-on-a-chip technology with advanced
analytical techniques and computational models aiming to transcend the
limitations of animal testing, especially in drug development and
toxicity studies. By leveraging these cutting-edge tools, we anticipate
achieving a deeper understanding of drug behavior, leading to the
identification and development of safer and more effective therapeutics,
ultimately benefiting human health and transforming the landscape of
pharmaceutical research.