Fig 3. Morphologic and differential characterization of the
intestinal epithelium on the gut-on-a-chip platform. (A) The
longitudinal section of chip and the immunofluorescence images of cells
cultured in gut-on-a-chip with fluidic force, in which expression of
ZO-1 (red) at different height could be observed. Corresponding to the
schematic, I represents apical side and sequentially V represents basal
side. Besides, nuclei are dyed by Hoechst (blue) while F-actin is marked
by phalloidin (green), the same below. (Scale bar: 50 μm). (B)Cross-sectional view of expression of ZO-1 in static chip (top) and
perfused chip (below). (Scale bar: 100 μm). (C) The integral 3D
image of intestinal cells layer in static chip (top) and perfused chip
(below). (D) Expression of MUC-2 in perfused chip (Scale bar:
50 μm). (E) The height of villi measured by Image J according
to the side view of immunofluorescence. (F&G)Cross-sectional view of expression
of Villin and Ki67 in static chip (F) and perfused chip (G) (Scale bar:
50 μm).