Results

Optimization of RT-LAMP settings for SARS-CoV-2 amplification

To determine the optimal RT-LAMP primer sets for SARS-CoV-2 we used isolated RNA from 30 clinical samples previously confirmed positive by qRT-PCR. A gradient of temperatures ranging from 60 to 65°C were assayed for end-point RT-LAMP reactions using RNA from viral hCoV-229E as negative control.