Results
Optimization of RT-LAMP settings for SARS-CoV-2
amplification
To determine the optimal RT-LAMP primer sets for SARS-CoV-2 we used
isolated RNA from 30 clinical samples previously confirmed positive by
qRT-PCR. A gradient of temperatures ranging from 60 to 65°C were assayed
for end-point RT-LAMP reactions using RNA from viral hCoV-229E as
negative control.