Conclusions
The combination of RT-LAMP and colorimetric detection with
DNA-nanoprobes developed in this study has led to a final plasmonic
biosensor easy to perform in resource-limited settings, and has proven
to be fast, reliable, and accurate. Furthermore, the presented platform
exhibits considerable potential for development as a Point-of-Care (POC)
diagnostic system through the utilization of a partial lysis buffer to
facilitate accessibility to viral RNA. Since it can be readily
implemented for different pathogens as SARS-CoV-2 and aMPV, our approach
will help to improve access to cost-effective diagnostic devices for the
massive screening in situ of, at least, most of the RNA-based
viruses of clinical or veterinary concern. Moreover, as colorimetric
results can be acquired by a conventional plate reader equipped with a
540 nm filter, this system could be also implemented in clinical
laboratories for routine molecular analysis.
Conflicts of Interest: The authors declare that the research
was conducted in the absence of any commercial or financial
relationships that could be construed as a potential conflict of
interest.
Author Contributions: Conceptualization, RM; investigation, PC,
SAL, RM; writing-original draft preparation, PC, SAL, LB, and RM;
writing-review and editing, PC, SAL, AD, EGL, LB, and RM; project
administration, LB and RM; supervision, LB and RM.; funding acquisition,
LB and RM. All authors have read and agreed to the published version of
the manuscript.
Funding: This research was supported by the State Research
Agency of the Spanish Government, grant PID2020-114956GB-100. SAL and PC
were initially supported by IND2017/BMD-7683 and IND2019/BIO-17124,
respectively, from Autonomous Community of Madrid within Industrial PhD
grant. PC was also supported by PAIT08/21-02/2021-08 contract, as well
as the current Investigo Program CT19/23-INVM-19 from MICINN (Spain)
Acknowledgments: We deeply acknowledge the Center for Avian
Health of Cataluña and Aragón (CESAC) and BioAssays for their strong
support and assistance in accessing diverse resources used in the study.