2.5 Antitumor activity test
The lung cancer A549 cells in the logarithmic growth phase state were
taken and adjusted to 1 × 105 cells/mL, and 100 μL
cells per well were added into a 96-well plate. At the same time, the
blank group and the normal cell control group were cultured overnight at
37 ℃, 5% CO2. The positive drug (adriamycin) and the
crude extract were dissolved and filtered in dimethylsulfoxide (DMSO).
Then, the cells were treated with different concentration gradients of
adriamycin and the crude extract for 48 h, respectively. MTT (20 μL) was
added to each well and incubated at 37 ℃ for 4 h. After adding 150 μL
DMSO, the absorbance OD490 was determined by a
microplate reader (BIOBRI, Chengdu, China).