2.4 Preparation of strain crude extract
The concentrations of conidia were adjusted to 1×106, and then 4 mL of each was added to 500 mL of liquid Sabourand medium. Then, fermentation was carried out by cultivation at 180 rpm on a shaker at 28 °C for 7 days. After fermentation, fluids were extracted using ethyl acetate (proportion 1:1) three times, while the mycelia were stored at -80 °C after quick freezing with liquid nitrogen. The extracts were dried in a rotary evaporator and dissolved in 1 mL of dimethyl sulfoxide. The crude extracts were used in subsequent cellular assays and analyzed the antitumor activity substances with the metabolomic assay. The metabolomic assay was entrusted to BaySpectrum Biologicals (https://www.bioprofile.cn).