2.4 Preparation of strain crude extract
The concentrations of conidia were adjusted to 1×106,
and then 4 mL of each was added to 500 mL of liquid Sabourand medium.
Then, fermentation was carried out by cultivation at 180 rpm on a shaker
at 28 °C for 7 days. After fermentation, fluids were extracted using
ethyl acetate (proportion 1:1) three times, while the mycelia were
stored at -80 °C after quick freezing with liquid nitrogen. The extracts
were dried in a rotary evaporator and dissolved in 1 mL of dimethyl
sulfoxide. The crude extracts were used in subsequent cellular assays
and analyzed the antitumor activity substances with the metabolomic
assay. The metabolomic assay was entrusted to BaySpectrum Biologicals
(https://www.bioprofile.cn).