2.5 Antitumor activity test
The lung cancer A549 cells in the logarithmic growth phase state were taken and adjusted to 1 × 105 cells/mL, and 100 μL cells per well were added into a 96-well plate. At the same time, the blank group and the normal cell control group were cultured overnight at 37 ℃, 5% CO2. The positive drug (adriamycin) and the crude extract were dissolved and filtered in dimethylsulfoxide (DMSO). Then, the cells were treated with different concentration gradients of adriamycin and the crude extract for 48 h, respectively. MTT (20 μL) was added to each well and incubated at 37 ℃ for 4 h. After adding 150 μL DMSO, the absorbance OD490 was determined by a microplate reader (BIOBRI, Chengdu, China).