2.5. Screening of Archaeal Strains for Salt, pH and Temperature Tolerance Ability
Archaeal strains were grown in the presence of different salt concentrations (2.0-5.0M NaCl), pH ranges 4-12 and temperature range 4-56 ℃ by using MGM broth medium. Strains were cultured in 250 ml flasks with continuous rotatory agitation at 150 rpm for 72 h. During incubation, archaeal growth was measured at different time intervals (3, 6, 12, 24, 48 and 72 h) by observing their OD at 600 nm.