2.8. Genome Annotation of Halorubrum lacusprofundiHL1RP11 and Halobacterium noricense NRS2HaP9
The de novo gene prediction was performed by using GeneMarks and CLC genomics workbench [41]. The functional classification was conducted through COG (corresponding cluster of orthologous groups of protein) analysis. The gene function was annotated by BLAST against Kyoto Encyclopedia of Genes and Genomes database KEGG pathway [42]. KEGG Orthology Based Annotation System (KOBAS 2.0) was used for functional analysis of genes. To predict genes and operons involved in secondary metabolism and antibiotic resistance antiSMASH 4.0 software was used [43]. The whole genome sequences of strains HL1RP11 and NRS2HaP9 were deposited in the GenBank database under the accession number JAJNEG000000000 and JAJSOI000000000.
Results