2.4. Sequencing and Phylogenetic Analysis
Amplified gene of 16S rRNA was sequenced to identify the archaeal genera and species. The sequences were submitted to NCBI. Based on results obtained by BLAST sequence, A phylogenetic tree was constructed by neighbor-joining method with the help of software MEGA 10 [33]. Test of phylogeny was done by using bootstrap method (1000 replicates). The 16S rRNA sequences of bacterial strains were deposited in the GenBank with accession numbers LT634693- LT634709.