2.7. DNA Isolation, Genome Sequencing and Assembly ofHalorubrum lacusprofundi HL1RP11 and Halobacterium noricense NRS2HaP9
Based on the results of plant growth promoting traits, two archaeal strains Halorubrum lacusprofundi HL1RP11 and Halobacterium noricense NRS2HaP9 were selected for whole genome sequence analysis and identification of PGP-related genes. These strains were grown into 100 mL MGM broth overnight at 37 °C with 125 rpm. The Genomic DNA was isolated using Genomic DNA isolation kit (Thermo Scientific GeneJET, USA). Genome sequencing of these haloarchaeal strains was performed with the Illumina HiSeq2000® sequencing platform. For the current study, 5 µg of genomic DNA was extracted from each archaeal strain and was prepared for genome sequencing using the Illumina HiSeq2000® library preparation kit (Illumina, Inc.), following the manufacturer’s instructions. The sequencing data was then assembled into complete contigs with SPAdes assembler Version 3.13.0 [39]. The contigs were arranged against the genomes of Halorubrum lacusprofundi ATCC 49239 andHalobacterium noricense A1 by using Mauve [40].