2.8. Genome Annotation of Halorubrum lacusprofundiHL1RP11 and Halobacterium noricense NRS2HaP9
The de novo gene prediction was performed by using GeneMarks and CLC
genomics workbench [41]. The functional classification was conducted
through COG (corresponding cluster of orthologous groups of protein)
analysis. The gene function was annotated by BLAST against Kyoto
Encyclopedia of Genes and Genomes database KEGG pathway [42]. KEGG
Orthology Based Annotation System (KOBAS 2.0) was used for functional
analysis of genes. To predict genes and operons involved in secondary
metabolism and antibiotic resistance antiSMASH 4.0 software was used
[43]. The whole genome
sequences of strains HL1RP11 and NRS2HaP9 were deposited in the GenBank
database under the accession number JAJNEG000000000 and JAJSOI000000000.
Results