2.4. Sequencing and Phylogenetic Analysis
Amplified gene of 16S rRNA was sequenced to identify the archaeal genera
and species. The sequences were submitted to NCBI. Based on results
obtained by BLAST sequence, A phylogenetic tree was constructed by
neighbor-joining method with the help of software MEGA 10 [33]. Test
of phylogeny was done by using bootstrap method (1000 replicates). The
16S rRNA sequences of bacterial strains were deposited in the GenBank
with accession numbers LT634693- LT634709.