2.7. DNA Isolation, Genome Sequencing and Assembly ofHalorubrum lacusprofundi HL1RP11 and Halobacterium
noricense NRS2HaP9
Based on the results of plant growth promoting traits, two archaeal
strains Halorubrum lacusprofundi HL1RP11 and Halobacterium
noricense NRS2HaP9 were selected for whole genome sequence analysis and
identification of PGP-related genes. These strains were grown into 100
mL MGM broth overnight at 37 °C with 125 rpm. The Genomic DNA was
isolated using Genomic DNA isolation kit (Thermo Scientific GeneJET,
USA). Genome sequencing of these haloarchaeal strains was performed with
the Illumina HiSeq2000® sequencing platform. For the current study, 5 µg
of genomic DNA was extracted from each archaeal strain and was prepared
for genome sequencing using the Illumina HiSeq2000® library preparation
kit (Illumina, Inc.), following the manufacturer’s instructions. The
sequencing data was then assembled into complete contigs with SPAdes
assembler Version 3.13.0 [39]. The contigs were arranged against the
genomes of Halorubrum lacusprofundi ATCC 49239 andHalobacterium noricense A1 by using Mauve [40].