2.5. Screening of Archaeal Strains for Salt, pH and Temperature
Tolerance Ability
Archaeal strains were grown in the presence of different salt
concentrations (2.0-5.0M NaCl), pH ranges 4-12 and temperature range
4-56 ℃ by using MGM broth medium. Strains were cultured in 250 ml flasks
with continuous rotatory agitation at 150 rpm for 72 h. During
incubation, archaeal growth was measured at different time intervals (3,
6, 12, 24, 48 and 72 h) by observing their OD at 600 nm.