Preparation of fish and gyrodactylids
Gyrodactylids were obtained by periodically introducing
gyrodactylid-free goldfish to aquariums with G.
kobayashii -infected goldfish (Zhou et al. 2017). In addition to
the goldfish, fingerlings or immature individuals of 12 fish species
(Table 1) were transferred to the laboratory and separately stocked in
several 100-L aquariums each equipped with an aerator. To remove
ectoparasites, fish were treated with an appropriate concentration of
formalin or salt solution. After 30 days in the aquariums, 10
individuals of each species were anaesthetized with 0.02% MS-222
(tricaine methanesulfonate) and examined to confirm their
gyrodactylid-free status using a stereomicroscope.