Table 1 : Comparison between the reported literature on TMAO detection.
Here we report a method for assaying the enzyme trimethylamine monooxygenase in which the TMAO formed is directly quantified. The presence of trimethylamine interferes with the previously reported methods for TMAO detection (Chang et al. , 2021a). We overcome this issue by reacting residual trimethylamine using a derivatizing reagent iodoacetonitrile (IACN), that does not interfere with the assay (Hefni et al., 2021). Thus, our detection method becomes TMAO-specific in the enzymatic assay.