STANDARD M10 SARS-CoV-2 assay
In total, 600 μL of viral transport medium of the specimen was loaded into the sample chamber of the cartridge of STANDARD M10 SARS-CoV-2.8 After placing the cartridge in the STANDARD M10 instrument (SD biosensor), all RT-PCR processes were automatically performed within 1 h. STANDARD M10 SARS-CoV-2 targets theE gene and ORF1ab gene, and the test reports a positive result if both genes’ cycle thresholds (Ct) values are within 40.0 cycles. All experiments were performed according to the manufacturer’s instructions.