Figure 5. Specificity of the RT-RAA detection system. Fluorescence-time curves for the detection of nucleic acids from 21 different pathogens. The curves of (A) RSVA, (B) HIPV, (C) Flu A, (D) Flu B, (E) SARS-CoV-2 and(F) ADV detection systems, respectively.
3.4Pharyngeal swab samples testing
To evaluate the performance, 71 positive pharyngeal swabs (11 samples of RSVA, 5 samples of HPIV, 6 samples of Flu A, 30 samples of Flu B, 11 samples of SARS-CoV-2 and 8 samples of ADV, respectively) and 14 negative samples (not containing the six viruses) were extracted for nucleic acid and parallelly detected by both RT-RAA and RT-PCR. The established RT-RAA assay could accurately detect all positive samples as positive within 20 minutes (Figure 6), while all negative samples as negative (data not given). The parallel detection results of RT-RAA and RT-PCR were shown in Table S4, the positive and negative coincidence rates of the two methods were both 100%, but RT-RAA was more time-saving than RT-PCR while used a more portable instrument.