Figure 3. The relative position map of fluorescent probe and candidate primers in the conserved sequence screened for each virus. The optimal primers for each virus were shown in red. The numbers in parentheses represent relative positions within conserved sequence of (A) RSVA (GenBank accession no. KX655697.1: 4610-5503), (B) HPIV (GenBank accession no. MF554715.1: 82-1656), (C) Flu A (GenBank accession no. NC_002018.1: 1-1014), (D) Flu B, (GenBank accession no. CY018656.1:45-1727), (E)SARS-CoV-2, (GenBank accession no. OQ253304.1: 28214-29464), (F) ADV (GenBank accession no. MW816018.1:18385-21189).
Table 1. Primer and probe sequences of the screened respiratory pathogens.