Figure 3. The relative position
map of fluorescent probe and candidate primers in the conserved sequence
screened for each virus. The optimal primers for each virus were shown
in red. The numbers in parentheses represent relative positions within
conserved sequence of (A) RSVA (GenBank accession no. KX655697.1:
4610-5503), (B) HPIV (GenBank accession no. MF554715.1: 82-1656), (C)
Flu A (GenBank accession no. NC_002018.1: 1-1014), (D) Flu B, (GenBank
accession no. CY018656.1:45-1727), (E)SARS-CoV-2, (GenBank accession no.
OQ253304.1: 28214-29464), (F) ADV (GenBank accession no.
MW816018.1:18385-21189).
Table 1. Primer and probe
sequences of the screened respiratory pathogens.