1F, Forward primer; R, Reverse primer; P, Probe.
3.2Sensitivity evaluation
To evaluate the sensitivity of RT-RPA assay for six respiratory viruses, serial 10-fold dilutions of RNA/DNA standards were prepared, and fluorescence-time curves were obtained with a real-time RT-RAA fluorometric detector, The results were shown in Figure 4. The detection limits for these viruses were: 102 copies/mL for RSVA (4A), 102 copies/mL for HPIV (4B), 102 copies/mL for Flu A (4C), 103copies/mL for Flu B (4D), 102 copies/mL for SARS-Cov-2 (4E), and 102 copies/mL for ADV (4F), respectively.