Figure legends
Figure 1. Allergen-responsive B-cell populations in allergic
rhinitis (AR) and allergic asthma (AA) subjects during allergen
immunotherapy (AIT). (A) Allergen immunotherapy study set-up and dosing
schedule. (B) Allergen-responsive B-cell identification and
clustering-based phenotyping. (C) Cluster density plots and cluster 4
(LAP+ B cells) frequency in AR and AA groups during
AIT. (D) Cluster density plots and cluster 1&5 (CD27+memory B cells) frequency in AR and AA groups during AIT. * fdr
< 0.05
Figure 2. Allergen-responsive memory B-cell populations in
allergic rhinitis (AR) and allergic asthma (AA) subjects during allergen
immunotherapy (AIT). (A) Allergen-responsive memory B-cells selected
for FACS isolation for RNA-sequencing. (B) Molecular degree of
perturbation quantifying the heterogeneity between samples to indicate
overall changes in gene expression. (C) Gene set enrichment analysis
indicating increased (red dots) or decreased (blue dots) expression of
gene pathways compared to time point 0. Dot size indicated fold change.
(D) IgH mapping of B cell receptors.