Figure legends
Figure 1. Allergen-responsive B-cell populations in allergic rhinitis (AR) and allergic asthma (AA) subjects during allergen immunotherapy (AIT). (A) Allergen immunotherapy study set-up and dosing schedule. (B) Allergen-responsive B-cell identification and clustering-based phenotyping. (C) Cluster density plots and cluster 4 (LAP+ B cells) frequency in AR and AA groups during AIT. (D) Cluster density plots and cluster 1&5 (CD27+memory B cells) frequency in AR and AA groups during AIT. * fdr < 0.05
Figure 2. Allergen-responsive memory B-cell populations in allergic rhinitis (AR) and allergic asthma (AA) subjects during allergen immunotherapy (AIT). (A) Allergen-responsive memory B-cells selected for FACS isolation for RNA-sequencing. (B) Molecular degree of perturbation quantifying the heterogeneity between samples to indicate overall changes in gene expression. (C) Gene set enrichment analysis indicating increased (red dots) or decreased (blue dots) expression of gene pathways compared to time point 0. Dot size indicated fold change. (D) IgH mapping of B cell receptors.