Raman shift (cm-1) Assignment Method Comments Refs.
582/1272 ν4 PO43-/Amide III Integral Mineral-to-matrix ratio (Less susceptible to tissue orientation effects) [182]
960/1272 ν1 PO43-/Amide III Integral Mineral-to-matrix ratio (Strongly sensitive to laser polarization) [105]
1070/960 ν1 CO32-1 PO43- Integral Mineral crystallinity (Carbonate-to-phosphate ratio) [158]
1070/1675 ν1 CO32-/Amide I Integral Represents the labile type-B carbonate content present in the tissue matrix. (Carbonate-to-matrix ratio) [182]
1375/1272 δ(CH2)/Amide III Integral The relative amount of proteoglycans [231]
1410/1272 β(CH2)/Amide III Integral The relative amount of lipids [182]
950/960 ACP/ν1 PO43- Integral The relative content of immature hydroxyapatite [158]
955/960 OCP/ν1 PO43- Integral The relative content of immature hydroxyapatite [155]
1070/1103 ν1 CO32-1 CO32- Integral The relative content of A, B-type substitution [155]
1209/1181
ω(CH2)/ν(C-O-C)
Integral
Organic Matrix Properties (Hyp/Pro)
[231]
1070 ν1 CO32- Peak fitting Inversely correlated with the degree of mineral crystallinity and crystallite length (Full width at half maximum (FWHM)) [182]
960 ν1 PO43- Peak fitting Inversely correlated with the degree of mineral crystallinity and crystallite length (Full width at half maximum (FWHM)) [182]