Conclusion
Dot-ELISA has several advantages over commercially available ELISA kits, including simplicity of the procedure, no requirement of highly precise equipment such as an ELISA reader, ability to interpret results visually, low cost and high sensitivity and specificity. The test is also desired to be amenable to sample pooling to cover herd level screening through bulk samples which facilitates mass surveillance. The results of the present study demonstrate that dot-ELISA may provide a suitable alternative to plate ELISA for the serodiagnosis of GWFI at regional centres. The present study reports the first dot-ELISA based on rHyC of P. silenus which can support mass surveillance programs at the field level to achieve sustainable control of GWFI.