Production of rHyC antigen
The expression of rHyC protein was induced using 1 mM IPTG and culture
was allowed to grow at 37 °C with shaking at 200 rpm for 6 hr. Protein
was purified under denaturing condition using 8 M urea in phosphate
buffer. The homogeneity of purified fusion protein with molecular weight
of about 45 kDa was analysed in SDS-PAGE (Fig. 1 ). The
estimated concentration of purified rHyC protein was 94 µg/mL of elutes
after dialysis.