Fig. 3 Specificity and cross reactivity of developed dot-ELISA; Lane 1: Goat warble fly; Lane 2: Oestrosis; Lane 3: Haemonchosis; Lane 4: Fasciolosis; Lane 5: Amphistomosis; Lane 6: Coenurosis; Lane 7: Cryptosporidiosis; Lane 8: Paratuberculosis; Lane 9: Enterotoxaemia; Lane 10: Brucellosis.
A Cohen’s kappa index of 0.973 with Standard Error (SE) of 0.019 at very high significance level (P<0.001) was observed between the rHyC based dot-ELISA and microtitre plate indirect-ELISA with a percentage of agreement of 98.657% (Table 2 ).
Table 2. Evaluation of Cohen’s kappa for inter rater reliability with rHyC based indirect ELISA as reference standard.