Evaluation of Dot-ELISA
The diagnostic sensitivity and specificity of the assay was assessed with 109 known positive serum for goat warble fly disease and 92 true negative serum samples. The developed dot-ELISA provided good parameters with DSn of 97.3% and DSp of 95.8% (Table 1). The rHyC based dot-ELISA showed strong reactivity with positive serum of GWFI and no cross-reactivity was observed with positive sera of important parasitic and bacterial diseases of goats as listed earlier (Fig 3).
Table1. Diagnostic parameters of the optimized rHyC based dot-ELISA for GWFI.