2.2. Dose selection, preparation, and mode of administration
Doxorubicin was dissolved in 0.9% normal saline, and a single dose of 90mg/kg body weight (BW) was intraperitoneally (IP) injected based on its success in inducing chromosomal damage in Wister rats (15).
In addition, DMF was prepared as a suspension using 5% tween-20 in double distilled water (DDW); where it was freshly prepared each day just before treatment and orally administered to rats by the utilisation of oral gavage as a single dose of 15mg/kg/day which was selected based on the previously-shown protective effect (16).