2.1. Chemicals
DMF (Purity >97%, CAS no.624-49-7) and Colchicine (Purity >95%, CAS no.64-86-8) were acquired from Sigma-Aldrich (St. Louis, MO 63103, USA). Doxorubicin (Doxorubicin HCl 50mg powder for injection, Khandelwal Labs, India) was purchased from local pharmacies. Fetal calf serum (FCS) was obtained from Capricon Scientific GmbH, South America, Giemsa stain (Sigma Chemicals, USA). Polysorbate 20 (Tween-20) was from Sinopharm chemical reagent Co., Ltd, China. All solvents and chemicals used were of analytical grade.