3.2. Effect of DMF on Mitotic index (MI) and Chromosomal Aberrations (CAs)
Table 1 showed that in Group II [rats orally administered DMF alone (15mg/kg/day)], there was a non-significant difference in the frequency of TCAs (P >0.05) in compassion toGroup I (control).
Furthermore, rats IP injected with a single dose of DOX (90mg/kg) (Group III ) exhibited a significant increase(P <0.05) in the frequency of all structural CAs types, total chromosome aberrations, and abnormal metaphases compared to the control (Group I) rats.
However, in Group IV (DMF pre-treatment prior to DOX (90mg/kg) exposure), there were significant reductions (P <0.05) in the frequency of TCA, chromatid break, ring formation, and chromosomal break compared to such frequency values in Group III , with no significant change in the chromatid and chromosomal gap appearance, chromosomal fragment deletion, acentric and dicentric chromosome when compared to (Group III ) rats.
Concerning the mitotic index (MI), table 1 showed that there was a non-significant difference in the percentage of MI (P >0.05) in Group II rats (DMF alone) when compared to the corresponding index in control (Group I ). Moreover, in rats IP-injected with a single dose of DOX (Group III ) there was a significant decrease (P <0.05) in the MI value compared to the corresponding index in control (Group I ).
However, in Group IV (DMF pre-treatment prior to DOX exposure), there was a significant increase (P <0.05) in MI compared to the corresponding index in Group III ( DOX only).