2.6 Investigating the role of IL-10 in SFTSV infected
THP-1-derived macrophages.
To investigate the role of IL-10 in SFTSV-infected THP-1 cells, THP-1
cells were infected with SFTSV at a MOI of 1, and SFTSV-infected THP-1
cells were divided into two different treatment groups: SFTSV-infected
THP-1 cells were used as the control group, and SFTSV-infected THP-1
cells were treated with the IL-10RA polyclonal antibody (10 μg/ml)
(Invitrogen). The two different treatment groups were incubated for 6,
12, 24 and 48 h, and the levels of IL-2, IL-4, IL-6, IL-10, IL-17A,
TNF-α, and IFN-γ were measured using human Th1/Th2/Th17 CBA kits (BD
Bioscience) according to the manufacturer’s instructions, with minor
modifications. Sample acquisitions were performed with a FACS Canto II
flow cytometer and analysed by FCAP Array software version 3.0 (BD
Bioscience). TGF-β was measured in the collected supernatants using a
TGF-β-1 Human ELISA Kit (Thermo Fisher Scientific) according to the
manufacturer’s protocols.[7. 18]