2.6 Investigating the role of IL-10 in SFTSV infected THP-1-derived macrophages.
To investigate the role of IL-10 in SFTSV-infected THP-1 cells, THP-1 cells were infected with SFTSV at a MOI of 1, and SFTSV-infected THP-1 cells were divided into two different treatment groups: SFTSV-infected THP-1 cells were used as the control group, and SFTSV-infected THP-1 cells were treated with the IL-10RA polyclonal antibody (10 μg/ml) (Invitrogen). The two different treatment groups were incubated for 6, 12, 24 and 48 h, and the levels of IL-2, IL-4, IL-6, IL-10, IL-17A, TNF-α, and IFN-γ were measured using human Th1/Th2/Th17 CBA kits (BD Bioscience) according to the manufacturer’s instructions, with minor modifications. Sample acquisitions were performed with a FACS Canto II flow cytometer and analysed by FCAP Array software version 3.0 (BD Bioscience). TGF-β was measured in the collected supernatants using a TGF-β-1 Human ELISA Kit (Thermo Fisher Scientific) according to the manufacturer’s protocols.[7. 18]