Primer design
For primer design, general primers for various aquaporin isoforms was followed since the aquaporin family is very extended in broccoli plants, counting with 65 members (Nicolas‐Espinosa and Carvajal, 2022), primer design was focused in evaluate the expression of the different subfamilies within PIP1 and PIP2. The primer design was carried out using Geneious prime 2020.1.2 software (https://www.geneious.com/), focused in conserved sites of the consensus sequences of PIP1-1, PIP1-2, PIP1-4, PIP1-5, PIP2-1, PIP2-2/3, PIP2-4, PIP2-5, and PIP2-7.
Table 1 . Primer set for aquaporin expression analyses.