Vesicle size
The average size of the two fractions of vesicles was analyzed using light-scattering technology; through intensity measurements with a Malvern ZetaSizer Nano XL machine (Malvern Instruments Ltd., Orsay, France), as previously described by Barrajón-Catalán et al. , (2010). This allowed the measurement of the particles with a size range from 1 nm to 3 µm.