Analysis of Mineral Nutrients
Leaves and roots were collected, dried, and ground into a fine powder dust. Then samples were digested in a microwave oven (CEM Mars Xpress, Stallings, NC, USA), by HNO3:HClO4 (2:1) digestion. The different elements were detected by a coupled plasma (ICP) analysis (Optima 3000, PerkinElmer, Waltham, MA, USA).