Figure 5. Transmission electronic microscopy images of membrane isolation from broccoli leaves. (A ) microsomal fraction (MF) with scale bar of 2 µm, (B ) MF, scale bar of 1 µm, (C ) plasma membrane (PM) with scale bar of 1 µm, and (D ) PM, scale bar of 500 nm, isolated from leaves of broccoli plants.
Figure 6 . Broccoli leaves aquaporin protein relative quantification via western-blot analysis of microsomal fraction (MF) (A-C ), and plasma membrane (PM), (D-F ). PIP1 (A and D ), PIP2 (B and E ), and PIP2-7 (C and F ) were analyzed in all treated plants representing control as 100%. Data is represented as means ± SE (n = 4). Different letters show statistical differences one-way ANOVA and Duncan test as post hoc (p < 0.05) .
Table 2 . Broccoli leaves sterol content of microsomal fraction (MF) and plasma membrane (PM) (in 125 μg of protein) from broccoli leaves under different stress treatments. Data is represented as means ± SE (n = 4). Different letters show statistical differences p < 0.05 with Duncan test as post hoc .