Primer design
For primer design, general primers for various aquaporin isoforms was
followed since the aquaporin family is very extended in broccoli plants,
counting with 65 members (NicolasāEspinosa and Carvajal, 2022), primer
design was focused in evaluate the expression of the different
subfamilies within PIP1 and PIP2. The primer design was carried out
using Geneious prime 2020.1.2 software
(https://www.geneious.com/),
focused in conserved sites of the consensus sequences of PIP1-1, PIP1-2,
PIP1-4, PIP1-5, PIP2-1, PIP2-2/3, PIP2-4, PIP2-5, and PIP2-7.
Table 1 . Primer set for aquaporin expression analyses.