The preparation of Bazhen decoction
The formula of Bazhen decoction is as the following: Panax ginseng 30g, Radix rehmanniae praeparata 30g, Radix paeoniae alba 30g, Ligusticum wallichii 30g, Poria cocos 30g, Angelica sinensis 30g, Rhizoma Atractylodis Macrocephalae 30g, Licorice 30g. The herbs were immersed in 800 mL distilled water, soaked for 3 hours, and boiled twice to get the water extract solution. The extracts were combined, filtered, and concentrated to 0.6 g/mL. The final solution was sonicated and centrifuged (3000rmp, 15min), the supernatant was collected, filtered with 0.45μM membrane, aliquot, and stored in -20°C.