FIGURE 3 DP scaffolds regulate macrophage polarization in
vitro.
(A) Viability of BMSCs seeded on each scaffold type at the
indicated time points. (B) Viability of RAW264.7 cells seeded
on each scaffold type at the indicated time points. (C) Flow
cytometric analysis of macrophage population expressing CD86 (M1 type)
and CD206 (M2 type) at 3 days after seeding. (D) mRNA levels of
Arg-1 and iNOS in macrophages on the 3rd day in
culture determined by qRT-PCR. (E) IL-10 and TNF-α levels in
the supernatants on the 3rd day in culture as detected
by ELISA. (F) Heatmap of the transcriptomes of RAW264.7 cells
after 3 days in culture on COL and DP scaffolds. (G) Volcano
plots of differentially expressed genes (DEGs) between the COL and DP
groups. (H) Gene Ontology (GO) enrichment analysis.(I) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis.