3.4 Effect of DP-induced macrophage polarization on BMSC migration
and differentiation in vitro
The effects of macrophages cultured on DP on the proliferation,
migration, and differentiation of BMSCs were further evaluated. CCK-8
analysis indicated no significant differences in cell proliferation
activity among the different groups (Figure 4A). Both wound scratch and
Transwell assays showed that BMSC migration was significantly promoted
by macrophages seeded on DP scaffolds in comparison to the different
control conditions (Figure 4B-E). Additionally, we observed that the CM
from macrophages cultured on DP facilitated the osteogenic
differentiation of BMSCs, as confirmed by ARS staining as well as
changes in the mRNA expression levels of Runx2 and BMP2 .
The obtained data indicate that CM from macrophages exposed to DP
promoted the migration and osteogenic differentiation of BMSCs.