FIGURE 5  In vivo bone regeneration with a rat critical-sized defect as a guided bone regeneration (GBR) model.
(A) Three-dimensional construction and representative micro-CT images for each scaffold group. Scale bars, 1 mm and 5mm. (B and C) Quantitative results for BMD and BV/TV. (D) Representative images of Masson trichrome staining of samples collected in the 8th week post-surgery. Scale bar, 200 μm. (E) Quantitative results for percentage of new bone growth. (F) Immunohistochemical staining for macrophage polarization pan marker CD68, M1 marker iNOS, and M2 marker CD206 in tissue filling the defect tissues. Positively stained cells are indicated by arrows. Scale bar, 100 μm. (G)Quantification of positively stained cells.