2.3. Intracerebroventricular drug administration
Mice were weighed and then anesthetized using pentobarbital sodium
before being implanted with a guide cannula for mice (RWD Life Science
Co., China) placed stereotaxically to extend 1 mm dorsal to the lateral
ventricle, as previously described. The cannula placed at 1.5 mm
lateral, 0.6 mm posterior, and 1.3 mm dorsal to the bregma. Following a
5-day recovery period after surgical implantation of the guide cannula,
individual mice received treatments in 5-μL injection doses into the
left lateral ventricle of the brain over 1 min followed by an additional
1-min delay to prevent liquid overflow using a 10-μL syringe at a rate
of approximately 5μL/ min.